Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase Guilherme Souza Prado , Pingdwende Kader Aziz Bamogo , Joel Antônio Cordeiro de Abreu , François-Xavier Gillet , Vanessa Olinto dos Santos , Maria Cristina Mattar Silva , Jean-Paul Brizard , Marcelo Porto Bemquerer , By expressing these plant-derived enzymes in combination with either bacterial halogenases and alternative substrates, we can … Here we use a transient expression system in the model plant Nicotiana benthamiana to reconstitute the two-step plant-derived biosynthetic pathway for auxin (indole acetic acid) to achieve accumulation up to 500 ng/g fresh mass (FM). This high α-AIC3 accumulation allowed its structural and functional characterizations. Agro-infiltration is an efficient and simple means of achieving transient expression in N. benthamiana. A construction harboring the green fluorescent protein (GFP) was added as a … Scholarly information regarding genetic modification in plants and particularly in tobacco to yield beneficial compounds is in plenty [ 27, 28 ]. View / Open Files. (A) DAB staining of N. benthamiana leaves expressed with GFP, PvAvh74, PvAvh74+DPI, or PvAvh74+PvAvh8. benthamiana (Azmi et al., 2018), although the transient expression of NLP1 caused severe necrotic lesions in N. benthamiana (Figure 1b,c ). Tg mice. Plants | Free Full-Text | A Putative Effector LtCSEP1 from ... nicotiana benthamiana, a wild relative of tobacco, has become increasingly popular as a heterologous expression platform for reconstituting plant natural product pathways, because it is amenable to agrobacterium- mediated transient expression, a scalable and highly flexible process that enables rapid expression of genes and enzymes from other … Kathleen M. Martin 1, Kristin Kopperud 1, Romit Chakrabarty 2, Rituparna Banerjee 1 +2 more. Nicotiana benthamiana, which is now widely used for transient expression assays in plants, is an alternative host which may circumvent the need for optimization required with microbial hosts.