Single-cell pseudo-temporal ordering Slingshot. analysis Pseudotime analysis with slingshot Loading the matrix. Slingshot was designed to model developmental trajectories in single-cell RNA sequencing data and serve as a component in an analysis pipeline after dimensionality … Techniques: Single-cell RNA-seq, Genomics, Gene Expression Analysis, Principal Component Analysis, RNA-seq. Slingshot: Cell lineage and pseudotime inference for single-cell transcriptomics Kelly Street1,8, Davide Risso2, Russell B. Fletcher 3, Diya Das , John Ngai3,6,7, Nir Yosef4,8, Elizabeth Purdom5,8, and Sandrine Dudoit 1,5,8 1Division of Biostatistics, School of Public Health, UC Berkeley. 2018) is a single-cell lineage inference tool, it can work with datasets with multiple branches. Pseudotime analysis tools. Single nuclei RNA-seq of mouse placental labyrinth development Monocle 3 0. This allows us to iden- Analysis In addition to explaining the increase in mucous cells, this change would help to explain the depletion in effector club. There are only 2 cells labeled ependymal. 使用轨迹推断（TI，trajectory inference）的方法可以根据测序的细胞之间表达模式的相似性对单细胞沿着轨迹进行排序，以此来模拟细胞动态变化的过程。.